Transcription Paves the Way Preparing Targets for V(D)J Recombinase:
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RAG Recombinase as a Selective Pressure for Genome Evolution
The RAG recombinase is a domesticated transposable element co-opted in jawed vertebrates to drive the process of the so-called V(D)J recombination, which is the hallmark of the adaptive immune system to produce antigen receptors. RAG targets, namely, the Recombination Signal Sequences (RSS), are rather long and degenerated sequences, which highlights the ability of the recombinase to interact w...
متن کاملV(D)J recombinase activity in common lymphoid progenitors
Common lymphoid progenitors (CLPs) are the first bone marrow precursors in which V(D)J recombinase activity is up-regulated. Here, we show that loss of the transcription factor E47 produces a reduced CLP population that lacks V(D)J recombinase activity and D-J H rearrangements in vivo. Apart from a profound arrest before the pro–B cell stage, other downstream lymphoid progeny of CLPs are still ...
متن کاملE47 is required for V(D)J recombinase activity in common lymphoid progenitors
Common lymphoid progenitors (CLPs) are the first bone marrow precursors in which V(D)J recombinase activity is up-regulated. Here, we show that loss of the transcription factor E47 produces a reduced CLP population that lacks V(D)J recombinase activity and D-J(H) rearrangements in vivo. Apart from a profound arrest before the pro-B cell stage, other downstream lymphoid progeny of CLPs are still...
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Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate the recombinase in multipotent hematopoietic progenitors. Using mice that express a fluorescent ...
متن کاملControl of Ig gene assembly: lessons from premature activation.
A new study by Bevington and Boyes reports that expression of the IRF4 transcription factor is sufficient to prematurely activate assembly of immunoglobulin light chain loci in pro-B cells. Using this in vivo model, they provide evidence that transcription-coupled disruption of nucleosome cores may be a key event in unlocking DNA substrates for recognition and assembly by V(D)J recombinase. Imm...
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تاریخ انتشار 2011